Jacalyn Kreitzer has sung with many opera houses and symphonies, including the Deutsche Oper Berlin, Théâtre du Chatelet, Theatre del Liceu, and the Lyric Opera of Chicago; the San Francisco, New York City, Geneva, Los Angeles, and Metropolitan operas; the St. Paul Chamber Orchestra; Minnesota Orchestra; and the Los Angeles and Pittsburgh symphonies, among others, under such conductors as Zubin Mehta, Loren Maazel, Valery Gerghiev and James Levine. At Carnegie Hall, she sang “Brangane” in Wagner’s Tristan und Isolde, with Loren Maazel conducting the Pittsburgh Symphony. She sang several times with Prague Radio Symphony, with James DePriest and Tamas Vasary conducting. Kreitzer sang the role of the “Mother” in Menotti’s The Consul on the Chandos label, recorded during performance in Spoleto, Italy, and has recorded with Chandos, Deutsche Grammophon, Claves and Teldec. She was the mezzo-soprano soloist in the San Luis Obispo Master Chorale’s performance of Verdi’s Requiem conducted by Thomas Davies in 2014, and in 2016 she was a soloist in the San Luis Obispo's performance of Mahler's Symphony No. 2, guest conducted by Davies.

Kreitzer is the founder of Cal Poly's Student Opera Theatre. She received the College of Liberal Arts Outstanding Lecturer award in 2002. In 2015 she was honored for outstanding achievement in the performing arts by ARTS Obispo. She is an Opera America Panelist.

Kreitzer retired the end of spring 2019.